AWS is the cloud-computing arm of online retailer Amazon.com.
Gamesa will supply 48 G114 2.1MW turbines to the Amazon Wind Farm US Central project, located in Paulding County, Ohio, northern US.
The US arm of Spanish developer EDP Renewables is developing the project.
Turbine delivery is expected in the second quarter of 2016, with commissioning due by the end of the year, according to Gamesa. The Spanish manufacturer said the deal takes orders for the G114 turbine to over 2GW.
The electricity produced from the project will power AWS's "current and future data centres".
AWS is looking to source 40% of the energy consumption from its global infrastructure from renewable energy by 2016, with a view to eventually achieving 100%.
In January 2015, AWS renamed the 150MW Fowler Ridge project in Indiana, as the Amazon Web Services Wind Farm. The project will use 65 Siemens 2.3MW turbines to power more of AWS's data centres.
In July AWS signed a power purchase agreement with Iberdrola at the 208MW Amazon Wind Farm US East project in North Carolina.