The new facility in Simoes Filhoe has the capacity to produce 100 AW3000 turbines a year, with the potential to increase this to up to 200.
Acciona said Brazil is the biggest market for the 3MW turbine.
The company said there was currently 210MW of the AW3000 operating in Brazil at fully commissioned projects. A further 201MW is in construction and 609MW "pending implementation".
It is the second manufacturing facility Acciona has opened in the Americas this year. In March, the Spanish multinational opened a concrete tower manufacturing plant near Monterrey, northeast Mexico.